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KMID : 0858420060080020204
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Korean Journal of Stroke
2006 Volume.8 No. 2 p.204 ~ p.208
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Angiogenic Effect of Delayed Erythropoietin Treatment in the Adult Rats with Focal Cerebral Ischemia
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Lee Kyung-Bok
Chu Kon
Sinn Dong-In
Park Dong-Kyu
Roh Jae-Kyu
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Abstract
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Background and Purpose: Among the extra-haematopoietic functions of erythropoietin (EPO), angiogenesis, a process through which new blood vessels arise from preexisting ones, has been indicated. We assessed the angiogenic effect of delayed EPO treatment in focal cerebral ischemic rat model.
Methods: Focal middle cerebral artery (MCA) infarctions were induced by intraluminal thread occlusion method for 90 minutes. Rats were divided into two groups；a control group that received saline only, and EPO injection group that received intraperitoneal EPO (5,000 IU/kg) injection at 3 days after MCA infarction for 4 days. All groups were injected with BrdU for 3 consecutive days at 7 days after MCA infarction. Immunohistochemical stainings with anti-laminin and antivon Willebrand factor (vWF) antibodies were performed to evaluate the amount of new vessel formation in ischemic boundary area. Quantitative measurement of angiogenesis and extent of ischemic injury was done after 10 days of focal ischemia.
Results: Vascular density in ischemic boundary was higher in the EPO injection group than the control group(2.67±0.51 mm2/microfield vs. 1.33±0.14mm2/microfield, p<0.001). Total number of vessels was increased in the EPO treated group than the control group (10.89±1.92 vs. 7.40±1.99, p<0.001) in vWF immunohistochemical staining. In the EPO group, many enlarged and thin-walled vessels were observed, and more vWF and BrdU double positive cells were seen in ischemic boundary. The infarction volume, however, was not different in both groups.
Conclusion: This study suggests that delayed EPO treatment enhance new vessel formation occurring in peri-infarct area after MCA infarction. (Korean Journal of Stroke 2006;8:204-208)
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KEYWORD
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Angiogenesis, Focal cerebral ischemia, Therapeutic angiogenesis
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